一、基本信息： 

姓名: 王东旭

性别: 男

出生日期: 1985.09.27

身体状况: 健康

婚姻状况: 已婚

手机: 18743089730

E-mail: wang_dong_xu@jlu.edu.cn

 

王东旭：男，博士生导师，长春国家实验动物中心/吉林大学动物科学学院教授，吉林省优秀青年基金获得者。一直致力于基因表达和动物模型的建立等方面的研究。拥有13年转基因动物模型经验，擅长肿瘤、慢性病疾病小鼠、兔及猪模型，累计建立20余种疾病动物模型。利用体细胞核移植和胚胎移植等技术，完成兔子、猪的孤雌模型构建。擅长基因编辑技术，借助CRISPR-Cas9和单碱基编辑系统等基因编辑技术已经成功构建多种基因编辑小鼠模型，如成骨不全基因编辑小鼠。利用单碱基突变技术，构建TETs蛋白家族点突变小鼠模型，获得吉林省科技厅实验动物平台项目支持。此外，还建立了肝癌小鼠模型、胃癌小鼠模型、肺癌小鼠模型、肝纤维化小鼠、舌鳞癌小鼠、神经退行性小鼠、黑色素瘤小鼠等10余种疾病模型。其中，构建的结直肠癌小鼠模型与长春汉兰多达公司合作，实现成果转化，完成110万元横向课题1项。主持国家青年自然基金1项，吉林省开放课题1项，吉林省优秀青年人才基金1项，中国博士后科学基金2项，吉林省教育厅“十三五”科学研究规划项目1项，吉林省科技发展计划项目1项。近五年来，在国际重要学术期刊发表SCI论文46篇，其中以第一作者发表论文5篇，并列第一作者20篇，通讯作者发表论文21篇。累计影响因子235.509。在教学上，讲授本科生《实验动物生产学》，《实验动物繁殖学》，《实验动物学》3门课程。

 

教育状况

2004–2008，就读于吉林大学畜牧兽医学院, 生物技术专业，获得学士学位

2009–2012，就读于吉林大学畜牧兽医学院, 生物化学与分子生物学专业，获得硕士学位

2012–2015，就读于吉林大学动物科学学院，生物化学与分子生物学专业，获得博士学位

2015–2018，就职于吉林大学动物科学学院，实验动物专业，讲师

2018–2023，就职于吉林大学动物科学学院，实验动物专业，副教授

2023-至今，就职于吉林大学动物科学学院，实验动物专业，教授

2019–至今，就职于吉林大学动物科学学院/长春国家实验动物中心，实验动物专业，博士生导师

承担的科技计划项目： 

国家重点研发计划项目“多基因修饰异种器官移植供体猪模型”（课题编号2022YFA1105404）2022-2027 子课题主持人

国家自然青年科学基金项目“猪孤雌发育中印迹基因H19/IGF2表达和甲基化模式的分析”（项目批准号：31601003），2017-2019 主持人

吉林省科技发展计划项目“小鼠肺癌模型中H19表达和甲基化模式的分析”（项目批准号：20170623093-05TC），2017-2019 主持人

中国博士后科学基金第 60 批面上资助项目“VPA/5-AZA调控H19/IGF2表达诱导细胞凋亡的分析”（项目批准号：2016M601384），2017-2019 主持人

中国博士后科学基金第 11 批特别资助项目“维生素C调控人肝癌细胞系中H19表达和甲基化模式分析”（项目批准号：2018T110250），2019-2021 主持人

吉林省科技发展计划项目优秀青年人才基金“小鼠孤雌胚胎发育中XIST表达和表观遗传修饰分析”（项目批准号：20190103071JH），2019-2021 主持人

 吉林省教育厅“十三五”科学研究规划项目“小鼠孤雌胚胎发育中H19表达模式的分析”（项目批准号：JJKH20200950KJ），2019-2021 主持人

汉达多兰公司“中药提取物治疗结直肠癌药物的研发”（项目批准号：2021220002000979），2021-2023 主持人

吉林省科技发展计划项目“TET蛋白家族单基因点突变小鼠模型的建立及应用”（项目批准号：212574TC010185619），2022-2024 主持人

吉林省发改委项目“用于促进糖尿病患者创口愈合的鹿茸干细胞外泌体水凝胶研发” （项目批准号：2023C028-6），2023-2024 主持人

专利

1.氨基酸序列小的蛋白序列MINI RFX-CAS13D

2.构建阿尔兹海默症的兔模型方法

3.基于CRISPR技术制备高产抗体兔的方法

4.利用eAID-Cas9构建的帕金森病兔模型及方法

5.使Cas3系统可以精确敲除的方法

6.提高多克隆兔抗体产生的方法

7.一种SOD1基因p.I151V突变的人源肌萎缩侧索硬化症兔模型及构建方法

8.一种RHOB基因点突变的人源脑瘫兔模型及构建方法

9.一种构建先天性高胰岛素血症模型的方法及模型

10.一种构建新型肌萎缩侧索硬化症模型的方法及模型

11.一种基于dCasRx-NSUN6单基因特异性M5C修饰编辑方法

12.一种能够实现单个AAV病毒包被的迷你碱基编辑器

13.一种人原发性小头畸形兔模型及构建方法

所获荣誉

2018年度，获得吉林大学优秀博士后

2019年度，获得吉林大学课堂教学质量奖

2020年度，入选吉林大学励新优秀青年教师培养计划

2020年度，获得第五届全国大学生生命科学创新创业大赛指导教师一等奖

2021年度，获得第六届全国大学生生命科学创新创业大赛指导教师一等奖

2021年度，获得吉林省高层次人才

2022年度，广东省众创杯科技海归领航赛银奖

2023年度，第七届创客广东三等奖

 

近年科研成果：

[1] Zhijing Yang, Weiwei Liu, Huimin Liu, Rong Li, Lu Chang, Shaoning Kan, Ming Hao, Dongxu Wang*, et al. The applications of polysaccharides in dentistry [J]. Frontiers in Bioengineering and Biotechnology, 2022, 10(970041. (Corresponding author) 6.064

[2] Qiang Feng, Dongxu Wang, Tianyi Xue, Chao Lin, Yongjian Gao, Liqun Sun, Ye Jin , Dianfeng Liu, et al. The role of RNA modification in hepatocellular carcinoma [J]. Frontiers in pharmacology, 2022, 13(984453. (Co-first authors) 5.988

[3] Huimin Liu, Dongxu Wang, Shaoning Kan, Ming Hao, Lu Chang, Pengxu Lu, Yangyang Liu, Ye Jin, Weiwei Liu, et al. The role of lncRNAs and XIST in oral cancer [J]. Frontiers in cell and developmental biology, 2022, 10(826650. (Co-first authors) 6.081

[4] Qirong Li, Dongxu Wang, Dayong Ding, Ye Feng, Ruizhi Hou, Dianfeng Liu, Chao Lin, Yongjian Gao, et al. The Role and Application of Exosomes in Gastric and Colorectal Cancer [J]. Frontiers in pharmacology, 2021, 12(825475. (Co-first authors) 5.988

[5] Xiaolan Li, Xiang Zhang, Ming Hao, Dongxu Wang, Ziping Jiang, Liqun Sun, Yongjian Gao, Ye Jin, Peng Lei, Yue Zhuo, et al. The application of collagen in the repair of peripheral nerve defect [J]. Frontiers in Bioengineering and Biotechnology, 2022, 10(973301. (Co-first authors) 6.064

[6] Huimin Liu, Dongxu Wang, Zhijing Yang, Shuangji Li, Han Wu, Jingcheng Xiang, Shaoning Kan, Ming Hao, Weiwei Liu, et al. Regulation of epigenetic modifications in the head and neck tumour microenvironment. Frontiers in Immunology. 2022;13:1050982. (Co-first authors) 8.786

[7] Chengshun Li, Dongxu Wang, Ziping Jiang, Yongjian Gao, Liqun Sun, Rong Li, Minqi Chen, Chao Lin, Dianfeng Liu, et al. Non-coding RNAs in diabetes mellitus and diabetic cardiovascular disease [J]. Frontiers in endocrinology (Lausanne), 2022, 13(961802. (Co-first authors) 6.055

[8] Ming Hao, MengNa Duan, Zhijing Yang, Hengzong Zhou, Shuangji Li, Jingcheng Xiang, Han Wu, Huimin Liu, Lu Chang, Dongxu Wang *, Weiwei Liu, et al. Engineered stem cell exosomes for oral and maxillofacial wound healing. Front Bioeng Biotechnol. 2022;10:1038261. (Corresponding author) 6.064

[9] Yidan Xia, Dongxu Wang, Yuting Piao, Minqi Chen, Duo Wang, Ziping Jiang, Bin Liu, et al. Modulation of immunosuppressive cells and noncoding RNAs as immunotherapy in osteosarcoma. Frontiers in Immunology. 2022;13:1025532. (Co-first authors) 8.786

[10] Zhijing Yang, Baozhen Sun, Jingcheng Xiang, Han Wu, Shaoning Kan, Ming Hao, Lu Chang, Huimin Liu, Dongxu Wang *, Weiwei Liu, et al. Role of epigenetic modification in interferon treatment of hepatitis B virus infection. Frontiers in Immunology. 2022;13:1018053. (Corresponding author) 8.786

[11] Qiang Feng, Baozhen Sun, Tianyi Xue, Rong Li, Chao Lin, Yongjian Gao, Liqun Sun, Yue Zhuo, Dongxu Wang *, et al. Advances in CAR T-cell therapy in bile duct, pancreatic, and gastric cancers. Frontiers in Immunology. 2022;13:1025608. (Corresponding author) 8.786

[12] Yidan Xia, Dongxu Wang, Da Liu, Jiayang Su, Ye Jin, Duo Wang, Beibei Han, Ziping Jiang, Bin Liu, et al. Applications of Chitosan and its Derivatives in Skin and Soft Tissue Diseases [J]. Frontiers in Bioengineering and Biotechnology, 2022, 10(894667. (Co-first authors) 6.064

[13] Huimin Liu, Yinyu Wang, Tianyi Xue, Zhijing Yang, Shaoning Kan, Ming Hao, Yang Gao, Dongxu Wang *, Weiwei Liu, et al. Roles of m6A modification in oral cancer (Review). International journal of oncology. 2023;62(1):5. (Corresponding author) 5.884

[14] Yidan Xia, Dongxu Wang, Jiaqi Li, Minqi Chen, Duo Wang, Ziping Jiang, Bin Liu, et al. Compounds purified from edible fungi fight against chronic inflammation through oxidative stress regulation [J]. Frontiers in pharmacology, 2022, 13(974794. (Co-first authors) 5.988

[15] Qirong Li, Dongxu Wang, Ziping Jiang, Rong Li, Tianyi Xue, Chao Lin, Yongzhi Deng, Ye Jin, Baozhen Sun, et al. Advances of hydrogel combined with stem cells in promoting chronic wound healing. Frontiers in chemistry. 2022;10:1038839. (Co-first authors) 5.545

[16] Yongjian Gao, Chengshun Li, Tianyi Xue, Chao Lin, Ruizhi Hou, Qianyun Xia, Dayong Ding, Jiaqi Li, Dongxu Wang *, Ye Feng, et al. Quercetin Mediated TET1 Expression Through MicroRNA-17 Induced Cell Apoptosis in Melanoma Cells [J]. Biochemical genetics, 2022, (Corresponding author) 2.220

[17] Lu Chang, Dongxu Wang, Shaoning Kan, Ming Hao, Huimin Liu, Zhijing Yang, Qianyun Xia, Weiwei Liu, et al. Ginsenoside Rd inhibits migration and invasion of tongue cancer cells through H19/miR-675-5p/CDH1 axis [J]. J Appl Oral Sci, 2022, 30(e20220144. (Co-first authors) 3.144

[18] Qirong Li, Baozhen Sun, Yue Zhuo, Ziping Jiang, Rong Li, Chao Lin, Ye Jin, Yongjian Gao, Dongxu Wang*, et al. Interferon and interferon-stimulated genes in HBV treatment. Frontiers in Immunology. 2022, 13:1034968. (Corresponding author) 8.786

[19] Chengshun Li, Xiaolan Li, Ziping Jiang, Dongxu Wang, et al. Flavonoids Inhibit Cancer by Regulating the Competing Endogenous RNA Network [J]. Frontiers in Oncology, 2022. (Co-first authors) 6.244

[20] Ming Hao, Dongxu Wang, Qianyun Xia et al. Pathogenic mechanism and multi-omics analysis of oral manifestations in COVID-19 [J]. Frontiers in Immunology,2022. (Co-first authors) 8.786

[21] Zhong-Hao Ji, Wen-Zhi Ren, Shuo Yang, Dongxu Wang, et al. Original Article Identification of immune-related biomarkers associated with tumorigenesis and prognosis in skin cutaneous melanoma[J]. Am J Cancer Res 2022;12(4):1727-1739. (Co-first authors) 6.166

[22] Chengshun Li, Ziping Jiang, Jindong Hao, Da Liu, Haobo Hu, Yan Gao, Dongxu Wang*, et al. Role of N6-methyl-adenosine modification in mammalian embryonic development [J]. Genetics and molecular biology, 2021, 44(2): e20200253. (Corresponding author) 2.087

[23] Lin Chen, Qirong Li, Ziping Jiang, Chengshun Li, Haobo Hu, Tiedong Wang, Yan Gao, Dongxu Wang*, et al. Chrysin Induced Cell Apoptosis Through H19/let-7a/COPB2 Axis in Gastric Cancer Cells and Inhibited Tumor Growth [J]. Frontiers in Oncology, 2021; 11:651644. (Corresponding author) 6.244

[24] Boqiang Tao, Dongxu Wang, Shuo Yang, Yingkun Liu, Han Wu, Zhanjun Li, Lu Chang, Zhijing Yang, Weiwei Liu*, et al. Cucurbitacin B Inhibits Cell Proliferation by Regulating X-Inactive Specific Transcript Expression in Tongue Cancer [J]. Frontiers in Oncology, 2021; 11:651648. (Co-first authors) 6.244

[25] Xinyi Zhang, Dianfeng Liu, Yongjian Gao, Chao Lin, Qingwu An, Ye Feng, Yangyang Liu, Da Liu, Haoming Luo*, Dongxu Wang*, et al. The Biology and Function of Extracellular Vesicles in Cancer Development [J]. Frontiers in Cell and Developmental Biology, 2021; 9:777441. (Corresponding author) 6.684

[26] Zhijing Yang, Da Liu, Hengzong Zhou, Boqiang Tao, Lu Chang, Huimin Liu, Haoming Luo, Dongxu Wang *, Weiwei Liu*, et al. A New Nanomaterial Based on Extracellular Vesicles Containing Chrysin-Induced Cell Apoptosis Through Let-7a in Tongue Squamous Cell Carcinoma [J]. Frontiers in Bioengineering and Biotechnology, 2021; 9:766380. (Corresponding author) 5.89

[27] Chengshun Li, Chuanni Peng , Ziping Jiang,  Haobo Hu , Chao Lin,  Yongjian Gao, Da Liu, Baozhen Sun *, Dongxu Wang *, et al. Ginkgo biloba Extract Inhibited Cell Proliferation and Invasion by Stimulating TET2 Expression Through miR-29a in Colorectal Carcinoma Cells [J]. DNA and Cell Biology, 2021. (Corresponding author) 3.311

[28] Qirong Li, Dongxu Wang, Dayong Ding, Feng Ye, Ruizhi Hou, Dianfeng Liu, Chao Lin, Gao Yongjian*, et al. The role and application of exosomes in gastric and colorectal cancer [J]. Frontiers in Pharmacology, 2021. (Co-first authors) 5.81

[29] Zhijing Yang, Dongxu Wang, Chenyu Zhang, Huimin Liu, Ming Hao, Shaoning Kan, Dianfeng Liu, Weiwei Liu*, et al. The applications of gold nanoparticles in the diagnosis and treatment of gastrointestinal cancer [J]. Frontiers in Oncology, 2021. (Co-first authors) 6.244

[30] Jindong Hao, Haobo Hu, Ziping Jiang, Xianfeng Yu, Chengshun Li, Lin Chen, Yidan Xia, Da Liu*, Dongxu Wang*, et al. microRNA-670 modulates Igf2bp1 expression to regulate RNA methylation in parthenogenetic mouse embryonic development [J]. Scientific Reports, 2020; 10(1):4782. (Corresponding author) 4.379

[31] Xiaowei Zhong, Siyi Huang, Dianfeng Liu, Ziping Jiang, Qinglong Jin, Chengshun Li, Liu Da, Qunyan Yao*, Dongxu Wang*, et al. Galangin promotes cell apoptosis through suppression of H19 expression in hepatocellular carcinoma cells [J]. Cancer Medicine, 2020; 9(15):5546-5557. (Corresponding author) 4.452

[32] Jindong Hao, Chengshun Li, Chao Lin, Yang Hao, Xianfeng Yu, Yidan Xia, Fei Gao, Ziping Jiang*, Dongxu Wang*, et al. Targeted point mutations of the m 6 A modification in miR675 using RNA-guided base editing induce cell apoptosis [J]. Bioscience Reports, 2020; 40(5). (Corresponding author) 3.84

[33] Xiaowei Zhong, Dianfeng Liu, Ziping Jiang, Chengshun Li, Lin Chen, Yidan Xia, Da Liu, Qunyan Yao*, Dongxu Wang*, et al. Chrysin induced cell apoptosis and inhibited invasion through regulation of TET1 expression in gastric cancer cells [J]. OncoTargets and Therapy, 2020; 13:3277-3287. (Corresponding author)4.147

[34] Wei Gao, Xianfeng Yu, Jindong Hao, Ling Wang, Minghui Qi, Liang Han, Chao Lin, Dongxu Wang*, et al. Ascorbic acid improves parthenogenetic embryo development through TET proteins in mice [J]. Bioscience Reports, 2019; 39(1). (Corresponding author) 3.84

[35] Jindong Hao, Yu Xianfeng, Wei Gao, Jiaqi Wei, Minghui Qi, Liang Han, Shuming Shi, Chao Lin, Dongxu Wang*, et al. The perturbed expression of m6A in parthenogenetic mouse embryos [J]. Genetics and Molecular Biology, 2019; 42(3):666-670. (Corresponding author) 1.771

[36] Xiaowei Zhong, Dianfeng Liu, Yang Hao, Chengshun Li, Jindong Hao, Chao Lin, Shuming Shi, Dongxu Wang*, et al. The expression of TET3 regulated cell proliferation in HepG2 cells [J]. Gene, 2019; 698:113-119. (Corresponding author) 1.771

[37] Yuning Song, Zhiquan Liu, Yang Han, Wei Gao, Jindong Hao, Xiaowei Zhong, Dongxu Wang*, Zhanjun Li*, et al. DNA methylation-mediated silencing of FLT1 in parthenogenetic porcine placentas [J]. Placenta, 2017; 58:86-89. (Corresponding author) 3.481

[38] Yang Hao, Guodong Wang, Chao Lin, Dong Li, Zhonghao Ji, Fei Gao, Zhanjun Li, Dianfeng Liu*, Dongxu Wang*, et al. Valproic acid induces decreased expression of H19 in A549 cells [J]. DNA and Cell Biology, 2017; 36(6):428-35. (Corresponding author) 3.311

[39] Dongxu Wang, Zhiquan Liu, Haobin Yao, Yang Hao, Lina Zhou, Jian Du, Yixin Zhu, Yuxin Xu, Guodong Wang, Yuning Song, Zhanjun Li*. Disruption of NNAT, NAP1L5 and MKRN3 DNA methylation and transcription in rabbit parthenogenetic fetuses [J]. Gene. 2017; 626:158-62. (First author) 3.688

[40] Dongxu Wang, Guodong Wang, Hao Yang, Haibo Liu, Cuie Li, Xiaolan Li, Chao Lin, Yuning Song, Zhanjun Li*, Dianfeng Liu, et al. DNA methylation modulate H19 and IGF2 expression in porcine female eye [J]. Genetics and Molecular Biology, 2017; 40(1):153-9. (First author)1.771

[41] Dongxu Wang, Lin Yuan, Tingting Sui, Yuning Song, Qingyan Lv, Anfeng Wang, Zhanjun Li*, Lai Liang-xue*, et al. Faithful Expression of Imprinted Genes in Donor cells of SCNT cloned pigs [J]. FEBS letters. 2015; 589; 2066-72. (First author) 4.124

[42] Dongxu Wang, Zhanjun Li*, Lai Liang-xue*, et al. Disruption of imprinted gene expression and DNA methylation status in porcine parthenogenetic fetuses and placentas [J]. Gene, 2014, 547(2): 351-8. (First author) 3.688

[43] Dongxu Wang, Zhanjun Li*, Lai Liang-xue*, et al. Genomic imprinting analysis of Igf2/H19 in porcine cloned fetuses using parthenogenetic somatic cells as nuclear donors [J]. Biotechnology letters, 2014, 36(10): 1945-52. (First author) 2.461

[44] Zhang Xiao-yang, Wang Dong-xu, Han Yang, et al. Altered imprinted gene expression and methylation patterns in mid-gestation aborted cloned porcine fetuses and placentas [J]. Journal of assisted reproduction and genetics, 2014;31(11):1511-7. (Co-first authors) 3.412

[45] Lin Chao, Liu Zeng-shan, Wang Dong-xu, et al. Sensitive and reliable micro-plate chemiluminescence enzyme immunoassay for okadaic acid in shellfish [J]. Anal Methods-Uk, 2014, 6(18): 7142-8. (Co-first authors) 2.896

[46] Li Xiao-lan, Song Nan, Wang Dong-xu, et al. (2015) Isoform-specific imprinting of the MEST gene in porcine parthenogenetic fetuses. Gene 558: 287-290. (Co-first authors) 3.688

总IF：235.509