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李占军

上传时间:2016-01-21点击率:43

 基本信息

姓 名 李占军

职 称 副教授

硕士生导师

所在部门 生物技术系

研究方向 克隆与基因修饰动物

办公电话 87836176

电子邮件 lizj_1998@jlu.edu.cn

二 学习与工作经历:

2014今 吉林大学动物科学学院 副教授 硕士生导师

2012—2014年, 吉林大学动物科学学院 讲师

2006—2012年, 吉林大学 动物学 博士

2009—2011年, UW-Madison医学院 联合培养博士

20022006年, 吉林大学 动物科学 学士

三 主要教学工作(承担的本科生、研究生课程)

本科生课程:《发育生物学》、《动物胚胎工程》。

研究生课程:《分子遗传学》、《动物胚胎工程》

四 主要科研工作(包括科研项目)

主要从事克隆与基因修饰动物,及基因编辑疾病动物模型的构建及药及机制研究。近年来发表SCI学术论文20余篇。主持或参与国家自然科学基金、博士后特别资助基金及转基因重大专项等科研项目。

承担科研项目

1、利用CRISPR/Cas9构建用于HIV-1研究的转基因兔模型博士后特别资助

2014.07-2015.07 15万 主持完成

2、广谱抗病毒转基因猪的制备博士后面上资助2013.09-2015.05 8万 主持完成

3、人源化基因修饰心房颤动猪模型的建立与转化横向课题2013.09-2015.09

20万 主持完成

4、猪孤雌胎儿发育阻滞的影响因素研究国家自然基金2013.01-2015.12

24万 主持完成

五 科研成果(著作、专利、论文)

发表的成果(论著)

发表SCI论文:

(1) Sui, Tingting(#)Yuan, Lin(#)Liu, HuanChen, MaoDeng, JichaoWang, YongLi, Zhanjun(*)Lai, Liangxue(*)CRISPR/Cas9 mediated mutation of PHEX in rabbit recapitulates human X-linked hypophosphatemia (XLH). Hum Mol Genet. 2016 Apr 28. pii: ddw125.

(2) Song, Yuning(#)Yuan, Lin(#)Wang, YongChen, MaoDeng, JichaoLv, QingyanSui, TingtingLi, Zhanjun(*)Lai, Liangxue(*)Efficient dual sgRNA directed large gene deletion in rabbit with CRISPR/Cas9 system.Cellular and Molecular Life Sciences2016 Jan 27.Epub ahead of print

(3) Lv, Qingyan(#)Yuan, Lin(#)Song, YuningSui, TingtingLi, Zhanjun(*)Lai, Liangxue(*)D-repeat in the XIST gene is required for X chromosome inactivationRNA Biology, 2016 May 15;582(2):122-7

(4)Lv, Qingyan(#)Yuan, Lin(#)Deng, JichaoChen, MaoWang, YongLi, Zhanjun(*)Lai, Liangxue(*)Efficient Generation of Myostatin Gene Mutated Rabbit by CRISPR/Cas9. Sci Rep. 2016 Apr 26;6:25029. 

(5) Yuan, Lin(#)Sui, Tingting(#)Chen, MaoDeng, JichaoHuang, YongyeZeng, JianLv, QingyanSong, YuningLi, Zhanjun(*)Lai, Liangxue(*)CRISPR/Cas9 mediated GJA8 knockout in rabbits recapitulates human congenital cataracts.Sci Rep2016622024-22024

(6) Lv,Qingyan(#)Lai,Liangxue(#)Yuan, LinSong, YuningSui, TingtingLi, Zhanjun(*)Tandem repeat knockout utilizing the CRISPR/Cas9 system in human cellsGene2016Epub ahead of print

(7) Song Yuning(#)Liangxue Lai(#)Li Li(#)Huang YongyeWang AnfengTang XiaochunPang DaxinLi Zhanjun(*)Ouyang Hongsheng(*)Germ cell-specific expression of Cre recombinase usingtheVASApromoter in the pigFEBS Open Bio2015112015)。

(8) Wang, Dongxu(#)Yuan, Lin(#)Sui, TingtingSong, YuningLv, QingyanWang, AnfengLi, Zhanjun(*)Lai, Liangxue(*)Faithful expression of imprinted genes in donor cells of SCNT cloned pigsFEBS Letters20155892015):2066-2072

(9) Duan, Feifei(#)Chen, Xianju(#)Yuan, Lin(#)Song, YuningWang, AnfengLv, QingyanLi, Zhanjun(*)Lai, LiangxueConservation of imprinting of Neuronatin (Nnat) in rabbitsSpringerPlus20154

(10) Li, Xiaolan(#)Song, Nan(#)Wang, Dongxu(#)Han, XiaoleiLv, QingyanOuyang, Hongsheng(*)Li, Zhanjun(*)Isoform-specific imprinting of the MEST gene in porcine parthenogenetic fetuses.Gene20152015 Mar 10558(2)):287-290

(11) Chen, Xianju(#)Wang, Tiedong(#)Lv, QingyanWang, AnfengOuyang, Hongsheng(*)Li, Zhanjun(*)DNA methylation-mediated silencing of neuronatin (NNAT) in pig parthenogenetic fetuses.Gene20145521):204-208

(12) Zhang, Xiaoyang(#)Wang, Dongxu(#)Han, YangDuan, FeifeiLv, QinyanLi, Zhanjun(*)Altered imprinted gene expression and methylation patterns in mid-gestation aborted cloned porcine fetuses and placentas.Journal of Assisted Reproduction and Genetics20143111):1511-1517

(13) Wang, Dongxu(#)Song, Yuning(#)Huang, Yongye(#)Duan, FeifeiLv, QinyanOuyang, HongshengLai, Liangxue(*)Li, Zhanjun(*)Genomic imprinting analysis of Igf2/H19 in porcine cloned fetuses using parthenogenetic somatic cells as nuclear donorsBiotechnology Letters20143610):1945-1952

(14) Wang, Dongxu(#)Chen, Xianju(#)Song, YuningLv, QinyanLai, LiangxueLi, Zhanjun(*)Disruption of imprinted gene expression and DNA methylation status in porcine parthenogenetic fetuses and placentas.Gene20145472):351-358

(15) Han, Yang(#)Ouyang, Hongsheng(#)Che, Dongsheng(#)Chen, XianjuHuang, Yongyeelhag, I. A. ElfakiPang, DaxinLi, Zhanjun(*)Elevated expression of vascular endothelial growth factor (VEGF) 120 in parthenogenetic porcine placentas.Biotechnology Letters2014365):913-917

(16) Yuan, Lin(#)Wang, Anfeng(#)Yao, Chaogang(#)Huang, YongyeDuan, FeifeiLv, QinyanWang, DongxuOuyang, HongshengLi, Zhanjun(*)Lai, Liangxue(*)Aberrant expression of Xist in aborted porcine fetuses derived from somatic cell nuclear transfer embryos.International Journal of Molecular Sciences20141512):21631-21643

(17) Han, Xiaolei(#)Ouyang, Hongsheng(#)Chen, Xianju(#)Huang, YongyeSong, YuningZhang, MingjunPang, DaxinLai, LiangxueLi, Zhanjun(*)Aberrant expression of Igf2/H19 in porcine parthenogenetic fetuses and placentas.Anim Reprod Sci20131391-4):101-108

(18) Huang, Yongye(#)Ouyang, Hongsheng(#)Yu, HaoLai, LiangxuePang, DaxinLi, Zhanjun(*)Efficiency of porcine somatic cell nuclear transfer - a retrospective study of factors related to embryo recipient and embryos transferred.Biol Open2013211):1223-1228

(19) Huang, Yongye(#)Li, Zhanjun(#)Wang, AnfengHan, XiaoleiSong, YuningYuan, LinLi, TianyeWang, Bing(*)Lai, Liangxue(*)Ouyang, Hongsheng(*)Pang, Daxin(*)Chimerism in piglets developed from aggregated cloned embryosFEBS Open Bio20162016 Mar 15;6(4):285-302. 

(20) Luo, Weiwei#(#)Li, Zhanjun#(#)Huang, YongyeHan, YangYao, ChaogangDuan, XinpingOuyang, Hongsheng(*)Li, Li(*)Generation of AQP2-Cre transgenic mini-pigs specifically expressing Cre recombinase in kidney collecting duct cells.Transgenic Research2014232):365-375

(21) Luo, Weiwei#(#)Li, Zhanjun#(#)Li, PengHuang, YongyeHan, YangYao, ChaogangZhang, ZhongzhanYan, HonggangPang, DaxinOuyang, HongshengLi, Li(*)Expression of Cre Recombinase in Alveolar Epithelial Cells of the AQP2-Cre Transgenic Mini-Pigs.Cellular Physiology and Biochemistry2014345):1597-1613

(22) Li, Zhanjun(#)Song, ChunhuaOuyang, HongshengLai, LiangxuePayne, Kimberly J.Dovat, Sinisa(*)Cell cycle-specific function of Ikaros in human leukemiaPediatric Blood and Cancer2012591):69-76