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欧阳红生

上传时间:2016-01-21点击率:77

 基本信息

姓 名 欧阳红生

职 称 教授

为博士生导师,硕士生导师

为吉林大学唐敖庆特聘教授

所在部门 动物生物技术系

研究方向 转基因动物培育,动物基因表达与调控,生物制药

办公电话 0431-87836175

电子邮件 ouyh@jlu.edu.cn

二 学习与工作经历:

1980-1984年,江西农业大学牧医系 兽医专业 农学学士

1984-1987,兽医大学,动物生物化学专业,农学硕士

1995-1999,农牧大学,生物化学与分子生物学专业,理学博士

1987-1989,兽医大学训练部生化教研室,助教

1989-1992,兽医大学基础部生化教研室,讲师

1992-1996,农牧大学基础部生化教研室,讲师

1996-1999,农牧大学基础部生化教研室,副教授

1999-2001,军需大学基础部生化教研室,副教授

2001-2002,军需大学兽医系生化教研室,教授

2001-2004,军需大学兽医系生化教研室主任,教授

2004-2012,吉林大学畜牧兽医学院动物生物技术系主任,教授

2013-至今,吉林大学动物科学学院副院长,教授

三 主要教学工作(承担的本科生、研究生课程)

本科生 生物化学

硕士生 分子遗传学

博士生 分子生物学

四 主要科研工作(包括科研项目)

1、抗病高产转基因猪新品种培育国家科技重大专项 2016ZX08006-003 2016.1-2020.12

2、抗猪瘟和腹泻转基因猪新品种培育国家科技重大专项 2014ZX08006-006 2014.1-2015.12

3、基于ipS细胞疾病模型猪的创制 973 2011CBA01003 2011.1-2015.12

4、抗病转基因猪新品种培育国家科技重大专项 2011ZX0806-001 2011.7-2012.12

五 科研成果(著作、专利、论文)

发表论文

1. Huang Y, Li Z, Wang A, Han X, Song Y, Yuan L, Li T, Wang B, Lai L, Ouyang H, Pang D. Chimerism in piglets developed from aggregated cloned embryos. FEBS Open Bio. 2016 Mar 15;6(4):285-302.

2. Liu X, Pang D, Yuan T, Li Z, Li Z, Zhang M, Ren W, Ouyang H, Tang X. N-3 polyunsaturated fatty acids attenuates triglyceride and inflammatory factors level in hfat-1 transgenic pigs. Lipids Health Dis. 2016 May 10;15(1):89.

3. Song Y, Lai L, Li L, Huang Y, Wang A, Tang X, Pang D, Li Z, Ouyang H. Germ cell-specific expression of Cre recombinase using the VASA promoter in the pig. FEBS Open Bio. 2015 Dec 29;6(1):50-55.

4. Nie D, Wang Z, Zhang Y, Pang D, Ouyang H, Li LI. Fat-1 gene inhibits human oral squamous carcinoma cell proliferation through downregulation of β-catenin signaling pathways. Exp Ther Med. 2016 Jan;11(1):191-196.

5. Peng Z, Ma T, Pang D, Su D, Chen F, Chen X, Guo N, Ouyang T, Ouyang H, Ren L. Expression, purification and antibody preparation of PCV2 Rep and ORF3 proteins. Int J Biol Macromol. 2016 May;86:277-281.

6. Ren L, Peng Z, Chen X, Ouyang H. Live Cell Reporter Systems for Positive-Sense Single Strand RNA Viruses. Appl Biochem Biotechnol. 2016 Apr;178(8):1567-1585.

7. Wang H, Song C, Ding Y, Pan X, Ge Z, Tan BH, Gowda C, Sachdev M, Muthusami S, Ouyang H, Lai L, Francis OL, Morris CL, Abdel-Azim H, Dorsam G, Xiang M, Payne KJ, Dovat S. Transcriptional Regulation of JARID1B/KDM5B Histone Demethylase byIkaros, Histone Deacetylase 1 (HDAC1), and Casein Kinase 2 (CK2) in B-cell Acute Lymphoblastic Leukemia. J Biol Chem. 2016 Feb 19;291(8):4004-18.

8. Li P, Luo W, Zhang H, Zheng X, Liu C, Ouyang H. Effects of Aluminum Exposure on the Bone Stimulatory Growth Factors in Rats. Biol Trace Elem Res. 2015 Nov 23.

9. Wang K, Ouyang H, Xie Z, Yao C, Guo N, Li M, Jiao H, Pang D. Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System. Sci Rep. 2015 Nov 13;5:16623.

10. Xu A, Li G, Yang D, Wu S, Ouyang H, Xu P, He F. Evolutionary Characteristics of Missing Proteins: Insights into the Evolution of Human Chromosomes Related to Missing-Protein-Encoding Genes. J Proteome Res. 2015 Dec 4;14(12):4985-4994.

11. Huang Y, Yuan L, Li T, Wang A, Li Z, Pang D, Wang B, Ouyang H. Valproic Acid Improves Porcine Parthenogenetic Embryo Development Through Transient Remodeling of Histone Modifiers. Cell Physiol Biochem. 2015;37(4):1463-1473.

12. Ruan J, Li H, Xu K, Wu T, Wei J, Zhou R, Liu Z, Mu Y, Yang S, Ouyang H,Chen-Tsai RY, Li K. Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs. Sci Rep. 2015 Sep 18;5:14253.

13. Tang X, Wang G, Liu X, Han X, Li Z, Ran G, Li Z, Song Q, Ji Y, Wang H, Wang Y, Ouyang H, Pang D. Overexpression of porcine lipoprotein-associated phospholipase A2 in swine. Biochem Biophys Res Commun. 2015 Sep 25;465(3):507-511.

14. Du X, Feng T, Yu D, Wu Y, Zou H, Ma S, Feng C, Huang Y, Ouyang H, Hu X, Pan D, Li N, Wu S. Barriers for Deriving Transgene-Free Pig iPS Cells with Episomal Vectors. Stem Cells. 2015 Nov;33(11):3228-3238.

15. Hu S, Qiao J, Fu Q, Chen C, Ni W, Wujiafu S, Ma S, Zhang H, Sheng J, Wang P, Wang D, Huang J, Cao L, Ouyang H. Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection. Elife. 2015 Jun 19;4:e06951.

16. Yang X, Ma T, Ouyang H, Chen F, Peng Z, Li C, Ma Y, Chen X, Li B, Pang D, Ren L. Effect of atovastatin treatment on porcine circovirus 2 infection in BALB/c mice. Clin Exp Pharmacol Physiol. 2015 Aug;42(8):817-21.

17. Li Z, Ren W, Han X, Liu X, Wang G, Zhang M, Pang D, Ouyang H, Tang X. ω3-polyunsaturated fatty acids suppress lipoprotein-associated phospholipase A2 expression in macrophages and animal models. Mol Nutr Food Res. 2015Sep;59(9):1771-1779.

18. Chen F, Jiang J, OuYang H, Ma T, Peng Z, Ma Y, Chen X, Pang D, Lin S, Ren L. Markerless Deletion System for Escherichia coli Using Short Homologous Sequences and Positive-Negative Selectable Cassette. Appl Biochem Biotechnol. 2015Jul;176(5):1472-1481.

19. Han X, Wang T, Zhang J, Liu X, Li Z, Wang G, Song Q, Pang D, Ouyang H, Tang X. Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NFκB pathways. Biol Open. 2015 Apr 2;4(5):661-665.

20. Chen F, Yang X, Pang D, Peng Z, Ma T, Ouyang H, Ren L. A dark-to-bright reporter cell for classical swine fever virus infection. Antiviral Res. 2015 May;117:44-51.

21. Li X, Song N, Wang D, Han X, Lv Q, Ouyang H, Li Z. Isoform-specific imprinting of the MEST gene in porcine parthenogenetic fetuses. Gene. 2015 Mar 10;558(2):287-290.

22. Wang D, Meng Q, Huo L, Yang M, Wang L, Chen X, Wang J, Li Z, Ye X, Liu N, Li Q, Dai Z, Ouyang H, Li N, Zhou J, Chen L, Liu L. Overexpression of Hdac6 enhances resistance to virus infection in embryonic stem cells and in mice. Protein Cell. 2015 Feb;6(2):152-156.

23. Yuan L, Wang A, Yao C, Huang Y, Duan F, Lv Q, Wang D, Ouyang H, Li Z, Lai L. Aberrant expression of Xist in aborted porcine fetuses derived from somatic cell nuclear transfer embryos. Int J Mol Sci. 2014 Nov 25;15(12):21631-21643.

24. Yan Q, Yang H, Yang D, Zhao B, Ouyang Z, Liu Z, Fan N, Ouyang H, Gu W, Lai L. Production of transgenic pigs over-expressing the antiviral gene Mx1. Cell Regen (Lond). 2014 Sep 26;3(1):11

25. Luo W, Li Z, Li P, Huang Y, Han Y, Yao C, Zhang Z, Yan H, Pang D, Ouyang H, Li L. Expression of Cre recombinase in alveolar epithelial cells of the AQP2-Cre transgenic mini-pigs. Cell Physiol Biochem. 2014;34(5):1597-613.

26. Chen X, Wang T, Lv Q, Wang A, Ouyang H, Li Z. DNA methylation-mediated silencing of neuronatin (NNAT) in pig parthenogenetic fetuses. Gene. 2014 Nov 15;552(1):204-208.

27. Wang H, Song C, Gurel Z, Song N, Ma J, Ouyang H, Lai L, Payne KJ, Dovat S. Protein phosphatase 1 (PP1) and Casein Kinase II (CK2) regulate Ikaros-mediated repression of TdT in thymocytes and T-cell leukemia. Pediatr Blood Cancer. 2014Dec;61(12):2230-2235.

28. Yang X, Ouyang H, Chen F, Ma T, Dong M, Wang F, Pang D, Peng Z, Ren L. Inhibition of 3-hydroxy-3-methylglutaryl-coenzyme A reductase increases the expression of interferon-responsive genes. Clin Exp Pharmacol Physiol. 2014Dec;41(12):950-955.

29. Cao Y, Ouyang H, Zhang M, Chen F, Yang X, Pang D, Ren L. Analysis of molecular variation in porcine reproductive and respiratory syndrome virus in China between 2007 and 2012. Virol Sin. 2014 Jun;29(3):183-188.

30. Wang D, Song Y, Huang Y, Duan F, Lv Q, Ouyang H, Lai L, Li Z. Genomic imprinting analysis of Igf2/H19 in porcine cloned fetuses using parthenogenetic somatic cells as nuclear donors. Biotechnol Lett. 2014 Oct;36(10):1945-1952.

31. Wang G, Ji Y, Li Z, Han X, Guo N, Song Q, Quan L, Wang T, Han W, Pang D,Ouyang H, Tang X. Nitro-oleic acid downregulates lipoprotein-associated phospholipase A2 expression via the p42/p44 MAPK and NFκB pathways. Sci Rep. 2014May 9;4:4905.

32. Lin S, Liang R, Meng X, OuYang H, Yan H, Wang Y, Jones GS. Construction and expression of mutagenesis strain of aroG gene from Escherichia coli K-12. Int J Biol Macromol. 2014 Jul;68:173-177.

33. Chen F, Yang X, Pang D, Peng Z, Dong M, Liu X, Ouyang H, Ren L. Expression, purification and antibody preparation using different constructs of PCV2 capsid protein. Int J Biol Macromol. 2014 Jun;67:289-94.

34. Wang H, Ouyang H, Lai L, Petrovic-Dovat L, Stankov K, Bogdanovic G, Dovat S. Pathogenesis and regulation of cellular proliferation in acute lymphoblastic leukemia - the role of Ikaros. J BUON. 2014 Jan-Mar;19(1):22-28.

35. Yang X, Ouyang H, Chen F, Pang D, Dong M, Yang S, Liu X, Peng Z, Wang F, Zhang X, Ren L. HMG-CoA reductase is negatively associated with PCV2 infection and PCV2-induced apoptotic cell death. J Gen Virol. 2014 Jun;95(Pt 6):1330-1337.

36. Han Y, Ouyang H, Che D, Chen X, Huang Y, Elfaki elhag IA, Pang D, Li Z. Elevated expression of vascular endothelial growth factor (VEGF) 120 in parthenogenetic porcine placentas. Biotechnol Lett. 2014 May;36(5):913-917.

37. Li X, Yang Y, Bu L, Guo X, Tang C, Song J, Fan N, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yi X, Quan L, Liu S, Yang Z, Ouyang H, Chen YE, Wang Z, Lai L. Rosa26-targeted swine models for stable gene over-expression and Cre-mediatedlineage tracing. Cell Res. 2014 Apr;24(4):501-504.

38. Zhao Y, Dai Z, Liang Y, Yin M, Ma K, He M, Ouyang H, Teng CB. Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system. Sci Rep. 2014 Feb 3;4:3943.

39. Ruan J, Liu N, Ouyang H, Yang S, Li K. Spatiotemporal control of porcine p65RHD expression by advanced Tet-On system in PIEC cells helps regulate NFкB activity. Mol Biol Rep. 2014 Mar;41(3):1753-1761.

40. Luo W, Li Z, Huang Y, Han Y, Yao C, Duan X, Ouyang H, Li L. Generation of AQP2-Cre transgenic mini-pigs specifically expressing Cre recombinase in kidney collecting duct cells. Transgenic Res. 2014 Apr;23(2):365-375.

41. Huang Y, Ouyang H, Yu H, Lai L, Pang D, Li Z. Efficiency of porcine somatic cell nuclear transfer - a retrospective study of factors related to embryo recipient and embryos transferred. Biol Open. 2013 Oct 2;2(11):1223-1228.

42. Yan S, Song H, Pang D, Zou Q, Li L, Yan Q, Fan N, Zhao X, Yu H, Li Z, Wang H, Gao F, Ouyang H, Lai L. Expression of plant sweet protein brazzein in the milk of transgenic mice. PLoS One. 2013 Oct 14;8(10):e76769.

43. Chen Y, Mei M, Zhang P, Ma K, Song G, Ma X, Zhao T, Tang B, Ouyang H, Li G, Li Z. The generation of transgenic mice with fat1 and fad2 genes that have their own polyunsaturated fatty acid biosynthetic pathway. Cell Physiol Biochem.2013;32(3):523-532.

44. Song N, Armstrong AD, Li F, Ouyang H, Niyibizi C. Multipotent mesenchymal stem cells from human subacromial bursa: potential for cell based tendon tissue engineering. Tissue Eng Part A. 2014 Jan;20(1-2):239-249.

45. Han X, Ouyang H, Chen X, Huang Y, Song Y, Zhang M, Pang D, Lai L, Li Z. Aberrant expression of Igf2/H19 in porcine parthenogenetic fetuses and placentas. Anim Reprod Sci. 2013 Jun;139(1-4):101-8.

46. Lin X, Yang X, Chen F, Cao Y, Meng X, Jiang J, Pang D, Zhao Y, Ouyang H, Ren L. Porcine CD4 promoters and enhancers can direct foreign gene expression in human cells. Biotechnol Lett. 2013 May;35(5):663-669.

47. Huang Y, Ouyang H, Xie W, Chen X, Yao C, Han Y, Han X, Song Q, Pang D, Tang X. Moderate expression of Wnt signaling genes is essential for porcine parthenogenetic embryo development. Cell Signal. 2013 Apr;25(4):778-785.

48. Fan N, Chen J, Shang Z, Dou H, Ji G, Zou Q, Wu L, He L, Wang F, Liu K, Liu N, Han J, Zhou Q, Pan D, Yang D, Zhao B, Ouyang Z, Liu Z, Zhao Y, Lin L, Zhong C, Wang Q, Wang S, Xu Y, Luan J, Liang Y, Yang Z, Li J, Lu C, Vajta G, Li Z, Ouyang H, Wang H, Wang Y, Yang Y, Liu Z, Wei H, Luan Z, Esteban MA, Deng H, Yang H, Pei D, Li N, Pei G, Liu L, Du Y, Xiao L, Lai L. Piglets cloned from induced pluripotent stem cells. Cell Res. 2013 Jan;23(1):162-166.

49. Yang X, Chen F, Cao Y, Pang D, Ouyang H, Ren L. Comparative analysis of different methods to enhance porcine circovirus 2 replication. J Virol Methods. 2013 Feb;187(2):368-371.

50. Yang X, Chen F, Cao Y, Pang D, Ouyang H, Ren L. Complete genome sequence of porcine circovirus 2b strain CC1. J Virol. 2012 Sep;86(17):9536.

51. Tan G, Niu J, Shi Y, Ouyang H, Wu ZH. NF-κB-dependent microRNA-125b up-regulation promotes cell survival by targeting p38α upon ultraviolet radiation. J Biol Chem. 2012 Sep 21;287(39):33036-33047.

52. Zheng X, Zheng W, Liu S, Patel HM, Xia X, Ouyang H, Levitt RC, Candiotti KA, Hao S. Crosstalk between JNK and NF-κB in the KDO2-mediated production of TNFα in HAPI cells. Cell Mol Neurobiol. 2012 Nov;32(8):1375-1383.

53. Lin S, Meng X, Jiang J, Pang D, Jones G, OuYang H, Ren L. Site-directed mutagenesis and over expression of aroG gene of Escherichia coli K-12. Int J Biol Macromol. 2012 Dec;51(5):915-919.

54. Ma X, Zhang P, Song G, Chen Y, Wang Z, Yin Y, Kong D, Zhang S, Zhao Z, Ouyang H, Tang B, Li Z. The construction and expression of lysine-rich gene in the mammary gland of transgenic mice. DNA Cell Biol. 2012 Aug;31(8):1372-1383.

55. Zheng W, Zheng X, Liu S, Ouyang H, Levitt RC, Candiotti KA, Hao S. TNFα and IL-1β are mediated by both TLR4 and Nod1 pathways in the cultured HAPI cells stimulated by LPS. Biochem Biophys Res Commun. 2012 Apr 20;420(4):762-767.

56. Zhu J, Pang D, Zhou Y, Tang X, Huang Y, Xie W, Gao F, Lai L, Zhang M, Ouyang H. Direct conversion of porcine embryonic fibroblasts into adipocytes by chemical molecules. Cell Reprogram. 2012 Apr;14(2):99-105.

57. Huo M, Chen N, Chi G, Yuan X, Guan S, Li H, Zhong W, Guo W, Soromou LW, Gao R, Ouyang H, Deng X, Feng H. Traditional medicine alpinetin inhibits the inflammatory response in Raw 264.7 cells and mouse models. Int Immunopharmacol.2012 Jan;12(1):241-248.

58. Li Z, Song C, Ouyang H, Lai L, Payne KJ, Dovat S. Cell cycle-specific function of Ikaros in human leukemia. Pediatr Blood Cancer. 2012 Jul 15;59(1):69-76.

59. Huang Y, Tang X, Xie W, Zhou Y, Li D, Yao C, Zhou Y, Zhu J, Lai L, Ouyang H, Pang D. Histone deacetylase inhibitor significantly improved the cloning efficiency of porcine somatic cell nuclear transfer embryos. Cell Reprogram. 2011Dec;13(6):513-520.

60. Wei J, Ouyang H, Wang Y, Pang D, Cong NX, Wang T, Leng B, Li D, Li X, Wu R, Ding Y, Gao F, Deng Y, Liu B, Li Z, Lai L, Feng H, Liu G, Deng X. Characterization of a hypertriglyceridemic transgenic miniature pig modelexpressing human apolipoprotein CIII. FEBS J. 2012 Jan;279(1):91-99.

61. Wu X, Ouyang H, Duan B, Pang D, Zhang L, Yuan T, Xue L, Ni D, Cheng L, Dong S, Wei Z, Li L, Yu M, Sun QY, Chen DY, Lai L, Dai Y, Li GP. Production of cloned transgenic cow expressing omega-3 fatty acids. Transgenic Res. 2012Jun;21(3):537-543.

62. Huang Y, Tang X, Xie W, Zhou Y, Li D, Zhou Y, Zhu J, Yuan T, Lai L, Pang D,Ouyang H. Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos. Biochem Biophys Res Commun. 2011 Jul29;411(2):397-401.

63. Tan G, Ren L, Huang Y, Tang X, Zhou Y, Zhou Y, Li D, Song H, Ouyang H, Pang D. Isolation and culture of embryonic stem-like cells from pig nuclear transfer blastocysts of different days. Zygote. 2012 Nov;20(4):347-352.

64. Li J, Dai Y, Liu S, Guo H, Wang T, Ouyang H, Tu C. In vitro inhibition of CSFV replication by multiple siRNA expression. Antiviral Res. 2011 Aug;91(2):209-216.

65. Peng L, Yanjiao M, Ai-guo W, Pengtao G, Jianhua L, Ju Y, Hongsheng O, Xichen Z. A fine balance between CCNL1 and TIMP1 contributes to the development of breast cancer cells. Biochem Biophys Res Commun. 2011 Jun 3;409(2):344-349.

66. Zhou Y, Ren L, Zhu J, Yan S, Wang H, Song N, Li L, Ouyang H, Pang D.Construction of a recombinant human FGF1 expression vector for mammary gland-specific expression in human breast cancer cells. Mol Cell Biochem. 2011Aug;354(1-2):39-46.

67. Wang H, Wan J, Wang W, Wang D, Li S, Liao P, Hao Z, Wu S, Xu J, Li N, Ouyang H, Gao H. Overexpression of Shadoo protein in transgenic mice does not impact the pathogenesis of scrapie. Neurosci Lett. 2011 May 27;496(1):1-4.

68. Zhao Y, Pang D, Wang T, Yang X, Wu R, Ren L, Yuan T, Huang Y, Ouyang H. Human MxA protein inhibits the replication of classical swine fever virus. Virus Res.2011 Mar;156(1-2):151-155.

69. Tang X, Guo Y, Nakamura K, Huang H, Hamblin M, Chang L, Villacorta L, Yin K, Ouyang H, Zhang J. Nitroalkenes induce rat aortic smooth muscle cell apoptosis via activation of caspase-dependent pathways. Biochem Biophys Res Commun. 2010Jun 25;397(2):239-244.

70. Li L, Pang D, Chen L, Wang T, Nie D, Yan S, Ouyang H. Establishment of a transgenic pig fetal fibroblast reporter cell line for monitoring Cre recombinase activity. DNA Cell Biol. 2009 Jun;28(6):303-308.

71. Li L, Pang D, Wang T, Li Z, Chen L, Zhang M, Song N, Nie D, Chen Z, Lai L, Ouyang H. Production of a reporter transgenic pig for monitoring Cre recombinase activity. Biochem Biophys Res Commun. 2009 May 1;382(2):232-235.

72. Li A, Sun Z, Ma Y, Yang F, Ouyang H, Yang X. The interaction of recombinant Pseudomonas aeruginosa exotoxin A with DNA and mimic biomembrane investigated by surface plasmon resonance and electrochemical methods. Talanta. 2006 Sep15;70(2):330-335.

获授权发明专利

1、体外转录调控DNA 201010182404.9 2012 中国

2、一种细胞信号通路抑制剂诱导猪脂记细胞形成的方法 201110404508.4 2013 中国

3、双温多重PCR鉴定猪早期胚胎性别的试剂盒 200910217772 2013 中国